The effect of dissolved oxygen partial pressure on the growth and carbohydrate metabolism of mouse LS cells.
نویسندگان
چکیده
Batch cultures of mouse LS cells were grown in suspension at controlled dissolved oxygen partial pressures (pOt). At low pOt (i-6 mmHg) the growth and respiration rates and the final cell population were all limited. At high pOt (320 mmHg), cell division was inhibited after an initial doubling of the cell number. At intermediate values of pOt, the growth rate was constant but the final cell population varied. Within the^>O2 range of 40-100 mmHg, the final cell population was constant and maximal at 1-2 x 10 viable cells/ml. Except at 320 mmHg pOt, about 90 % of the glucose consumed served as an energy source and could be accounted for as lactate and CO,. In the culture at 320 mmHg, only 60 % of the glucose consumed could be accounted for in this way. During growth the production of lactate and pyruvate was highest at low pOt. A sharp increase in lactate production was observed as logarithmic growth ceased in each culture, except at high pO% (160 mmHg). These observations indicate that pOt markedly influences cell growth and carbohydrate metabolism in these cells.
منابع مشابه
Effect of the selected physico-chemical parameters on growth of rainbow trout (Oncorhynchus mykiss) in raceway system in Iran
A study was conducted to evaluate the correlation between selected physico-chemical parameters of water and their effects on growth of Rainbow trout (Oncorhynchus mykiss) in raceway system in the area of Sarab Gerdu, Iran. The samples were collected from twenty raceways (270- day old fish, surface area 55m2 flow rate 150 ls-1 total production 15 T). Physico-chemical parameters were the fluctuat...
متن کاملEffect of the selected physico-chemical parameters on growth of rainbow trout (Oncorhynchus mykiss) in raceway system in Iran
A study was conducted to evaluate the correlation between selected physico-chemical parameters of water and their effects on growth of Rainbow trout (Oncorhynchus mykiss) in raceway system in the area of Sarab Gerdu, Iran. The samples were collected from twenty raceways (270- day old fish, surface area 55m2 flow rate 150 ls-1 total production 15 T). Physico-chemical parameters were the fluctuat...
متن کاملEffect of Dissolved Oxygen and Chemical Oxygen Demand to Nitrogen Ratios on the Partial Nitrification/Denitrification Process in Moving bed Biofilm Reactors
Partial nitrification was reported to be technically feasible and economically favorable, especially for wastewaterwith high ammonium concentration or low C/N ratio. In this study, the effect of dissolved oxygen (DO)and influent ratio of chemical oxygen demand to nitrogen (COD/N) ratio on biological nitrogen removal fromsynthetic wastewater was investigated. Experiments were c...
متن کاملThe effect of maternal hypothyroidism during pregnancy on carbohydrate metabolism in adulthood in rats
Introduction: Many of the diseases of adulthood are originated from the intrauterine conditions during fetal life. Because of the importance of thyroid hormones in growth and development of the fetus, the effects of maternal hypothyroidism on carbohydrate metabolism in adulthood was investigated. Methods: Pregnant rats were divided into the fetal hypothyroidism (FH) and the control (C) group...
متن کاملP-28: The Effect of Hydrostatic Pressure on the In vitro Maturation of Preantral Follicles Derived from Polycystic Ovaries in Mouse Model
Background: Polycystic ovary syndrome (PCOS) is a multifactorial, heterogeneous, complex genetic endocrine and metabolic disorder, diagnostically characterized by chronic anovulation, polycystic ovaries and biochemical and clinical manifestations of hyperandrogenism. In patients with PCOS lower apoptotic rates found in granulosa cells compared to women with regular ovulatory cycles and this lea...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of cell science
دوره 4 1 شماره
صفحات -
تاریخ انتشار 1969